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BIOT2215 – Biotechnology Methods I pdf

Credits: 4 (2/2/0)
Description: This course is the first in a two-semester sequence designed to train laboratory technicians in the fundamental technologies, procedures and processes utilized in the biotechnology industry. This course emphasizes technical skills development, record-keeping and communication skills, and conformity to good laboratory practices (GLPs) and good manufacturing practices (GMPs). This course meets for two hours of lecture and four hours of laboratory each week.
Prerequisites: BIOL1122 AND BIOL1115 AND CHEM1111
Corequisites: (None)
  1. Perform record-keeping, communication and technical skills that conform to industry standards.
  2. Demonstrate good laboratory practice (GLP) and good manufacturing practice (GMP) solution and media preparation skills.
  3. Demonstrate GLP and GMP cell culture skills, including stock culture preparation and storage.
  4. Describe the requirements for the polymerase chain reaction (PCR).
  5. Discuss the features of quantitative real-time PCR (qPCR).
  6. Explain restriction enzyme analysis.
  7. Discuss the features and applications of cloning vectors.
  8. Summarize techniques used to isolate and purify genomic and plasmid DNA.
  9. Discuss the requirements for DNA transfer, probe generation and DNA-DNA hybridization.
  10. Describe techniques used to isolate, purify and visualize RNA.
  11. Explain methods used to detect gene expression.
  12. Analyze experimental results.
  13. Troubleshoot experiments and equipment.
  14. Explore emerging trends in biotechnology and molecular diagnostics.
MnTC goal areas: (N/A)

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